7MJV
MiaB in the complex with s-adenosylmethionine and RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.155, 81.442, 109.751 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.510 - 2.240 |
R-factor | 0.2456 |
Rwork | 0.243 |
R-free | 0.27260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qgq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.887 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.240 | 4.850 | 2.250 |
Rmerge | 0.153 | 0.087 | 0.543 |
Rmeas | 0.164 | 0.093 | 0.600 |
Rpim | 0.058 | 0.031 | 0.250 |
Number of reflections | 22398 | 2561 | 1609 |
<I/σ(I)> | 7.6 | ||
Completeness [%] | 93.7 | 99.6 | 68.4 |
Redundancy | 7.3 | 8.4 | 4 |
CC(1/2) | 0.996 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM Na-cacodylate, pH 5.5, 13% PEG 4000 and 3% (+/-)-2-methyl-2,4-pentanediol |