7M96
Bovine sigma-2 receptor bound to Z4857158944
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-10-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033167 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.700, 55.350, 92.950 |
Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
Resolution | 40.190 - 2.410 |
R-factor | 0.2521 |
Rwork | 0.250 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7m93 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.577 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.200 | 2.550 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmeas | 0.223 | |
Number of reflections | 27659 | 4422 |
<I/σ(I)> | 5.18 | 0.56 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 4.4 | 4.5 |
CC(1/2) | 0.995 | 0.334 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 293 | PEG 300,0.1 M MES pH 6,560 mM ammonium phosphate |