7LTG

STRUCTURE OF HUMAN HDAC2 IN COMPLEX WITH APICIDIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	92.270, 98.390, 139.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.250 - 1.800
R-factor	0.17
Rwork	0.168
R-free	0.19800
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BUSTER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.360	1.831
High resolution limit [Å]	1.800	1.800
Number of reflections	116694	5751
<I/σ(I)>	8.4
Completeness [%]	99.2
Redundancy	6.2
CC(1/2)	0.995	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 3350, 0.2 M ammonium sulfate, 0.1 M Hepes pH 7.5