7LT5
CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Cofactor SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.418, 160.856, 230.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.100 - 2.540 |
R-factor | 0.1779 |
Rwork | 0.177 |
R-free | 0.21150 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7lnj |
RMSD bond length | 0.002 |
RMSD bond angle | 0.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 2.640 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.179 | 1.498 |
Rpim | 0.058 | 0.478 |
Number of reflections | 88557 | 8168 |
<I/σ(I)> | 10.7 | 1.4 |
Completeness [%] | 88.8 | 83.1 |
Redundancy | 9.1 | |
CC(1/2) | 0.564 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 292 | 0.1 M Tris-HCl pH 7.3, 23% PEG 3350, 0.27 M Potassium citrate |