7LLP
X-ray radiation damage series on Lysozyme at 277K, crystal structure, dataset 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2018-11-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88557 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.258, 77.258, 37.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.550 - 1.130 |
R-factor | 0.1327 |
Rwork | 0.132 |
R-free | 0.15460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.008 |
RMSD bond angle | 0.992 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.630 | 1.150 |
High resolution limit [Å] | 1.130 | 1.130 |
Rmerge | 0.072 | 2.720 |
Rpim | 0.026 | 0.989 |
Number of reflections | 42780 | 2047 |
<I/σ(I)> | 12.4 | 0.9 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 8.5 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Lysozyme was dissolved in 0.1 M Sodium Acetate pH 4.6 at 100 mg/ml and 5 microliters of this protein solution was mixed with 5 microliters of 0.1 M Sodium Acetate and 0.6 M Sodium Chloride solution pH 4.6. The 10 microliters drop was equilibrated against 0.7 milliliters of 2.2 M Ammonium Sulfate well solution. |