7LDK
Structure of human respiratory syncytial virus nonstructural protein 2 (NS2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 62 |
Unit cell lengths | 157.646, 157.646, 47.291 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.550 - 2.820 |
R-factor | 0.21394 |
Rwork | 0.212 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house Se-Met-labeled NS2 model solved by SAD phasing |
RMSD bond length | 0.010 |
RMSD bond angle | 1.589 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 2.154 |
Rmeas | 0.090 | 2.154 |
Rpim | 0.033 | 0.842 |
Number of reflections | 16685 | 740 |
<I/σ(I)> | 24.8 | 0.82 |
Completeness [%] | 99.4 | 91.1 |
Redundancy | 7.1 | 5.9 |
CC(1/2) | 0.995 | 0.432 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.4 M potassium-sodium-tartrate |