7KJT
KEOPS tRNA modifying sub-complex of archaeal Cgi121 and tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.284, 121.284, 91.734 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.580 - 3.340 |
| R-factor | 0.2158 |
| Rwork | 0.215 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3enh |
| Data reduction software | DIALS (1.14.5) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.620 | 60.620 | 3.430 |
| High resolution limit [Å] | 3.340 | 14.940 | 3.340 |
| Rmerge | 0.095 | 0.025 | 1.420 |
| Number of reflections | 11643 | 157 | 852 |
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 100.0 | 98.2 | 100 |
| Redundancy | 9.6 | 8.6 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, pH 7.5, 200 mM lithium sulfate, 25% PEG3000 |
