7KJO
crystal structure of PLEKHA7 PH domain biding SO4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.116 |
Spacegroup name | P 41 |
Unit cell lengths | 64.830, 64.830, 59.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.842 - 1.450 |
R-factor | 0.1539 |
Rwork | 0.153 |
R-free | 0.17140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1upr |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.842 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.050 | 0.362 |
Number of reflections | 46929 | 4283 |
<I/σ(I)> | 22.2 | |
Completeness [%] | 99.2 | |
Redundancy | 19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | protein in 180 mM NaCl and 20 mM sodium phosphate mixed with 25% glycerol, 2 M ammonium sulfate |