7KIO
Crystal structure of inositol polyphosphate 1-phosphatase (INPP1) D54A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS II |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1998-01-01 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 51.447, 51.447, 143.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.930 - 2.400 |
R-factor | 0.1936 |
Rwork | 0.191 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1inp |
RMSD bond length | 0.005 |
RMSD bond angle | 0.750 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.930 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.401 |
Number of reflections | 14352 | 1311 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 98.6 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium Citrate pH 4.3, 16-18% PEG6000, 5 mM CaCl2 |