7KHD
Human GITR-GITRL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 168.420, 168.420, 168.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.660 - 2.956 |
| R-factor | 0.219502182064 |
| Rwork | 0.218 |
| R-free | 0.25405 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.660 | 3.062 |
| High resolution limit [Å] | 2.956 | 2.956 |
| Number of reflections | 16802 | 1698 |
| <I/σ(I)> | 20.83 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.5 | |
| CC(1/2) | 0.999 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 30% PEG300, 0.2 M sodium sulfate decahydrate, and 0.1 M Adenosine-5-triphosphate disodium salt hydrate |
