7K3K
Crystal structure of dLC8 in complex with Panoramix TQT peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 45.639, 45.639, 202.717 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.794 - 1.415 |
R-factor | 0.1842 |
Rwork | 0.183 |
R-free | 0.20060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zke |
RMSD bond length | 0.005 |
RMSD bond angle | 0.806 |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.360 | 1.440 |
High resolution limit [Å] | 1.415 | 1.420 |
Rmerge | 0.069 | |
Number of reflections | 24954 | 999 |
<I/σ(I)> | 33.6 | |
Completeness [%] | 99.1 | |
Redundancy | 15.6 | |
CC(1/2) | 0.999 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M NH4Ac, 0.1 M Bis-Tris pH 5.5, 17% (w/v) PEG 10000 |