7JZC
Dihydrodipicolinate synthase S48W mutant with lysine in the allosteric site, and pyruvate in the catalytic site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 294.15 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 121.129, 121.129, 111.271 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.650 - 2.070 |
R-factor | 0.17412 |
Rwork | 0.172 |
R-free | 0.20861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yxc |
RMSD bond length | 0.020 |
RMSD bond angle | 2.010 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.340 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.086 | 0.329 |
Rpim | 0.039 | 0.156 |
Number of reflections | 57849 | 4524 |
<I/σ(I)> | 17.5 | |
Completeness [%] | 100.0 | |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294.15 | glucopyranoside, potassium phosphate dibasic |