7JQX
Crystal structure of Cfl1 wild-type from Burkholderia cenocepacia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9793 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 132.150, 132.150, 341.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.630 - 2.200 |
R-factor | 0.1817 |
Rwork | 0.180 |
R-free | 0.21230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kd2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.630 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.178 | |
Number of reflections | 76696 | 11911 |
<I/σ(I)> | 11.71 | |
Completeness [%] | 99.5 | |
Redundancy | 13.3 | |
CC(1/2) | 0.998 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 750 mM potassium sodium tartrate, 100 mM HEPES pH 8.1 |