7JLN
Crystal structure of glVRC01 Fab in complex with anti-idiotype iv9 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.420, 50.073, 125.171 |
Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
Resolution | 47.720 - 2.570 |
R-factor | 0.1998 |
Rwork | 0.197 |
R-free | 0.25570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mft |
RMSD bond length | 0.003 |
RMSD bond angle | 0.582 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.665 |
High resolution limit [Å] | 2.570 | 2.573 |
Number of reflections | 26793 | 2057 |
<I/σ(I)> | 7.92 | |
Completeness [%] | 99.8 | |
Redundancy | 3.2 | |
CC(1/2) | 0.981 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08 M Sodium Acetate, 24% PEG 4000, 0.16 M Ammonium Acetate |