7ICO

DNA POLYMERASE BETA (E.C.2.7.7.7)/DNA COMPLEX, SOAKED IN THE PRESENCE OF ZNCL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-01-23
Detector	SDMS
Spacegroup name	P 21 21 2
Unit cell lengths	179.143, 57.535, 48.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.156
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	9ick
RMSD bond length	0.020
RMSD bond angle	2.900
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	TNT (V. 5-D)
Refinement software	TNT (5-D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.075 *	0.153 *
Number of reflections	7323
Completeness [%]	91.0	88
Redundancy	2.0 *	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5		12-14% PEG3350, 0.1M HEPES, pH.7.5 and 50 mM LiSO4 can be used as reservoirs *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	10-12 (%(w/v))
2	1	reservoir	MES	0.1 (M)
3	1	reservoir		0.1 (M)