7FC3
structure of NL63 receptor-binding domain complexed with horse ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-19 |
Detector | DECTRIS PILATUS 12M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.905, 112.905, 328.924 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.230 - 3.190 |
R-factor | 0.235 |
Rwork | 0.232 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kbh |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.230 | 3.300 |
High resolution limit [Å] | 3.190 | 3.190 |
Number of reflections | 21422 | 2019 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 98.8 | |
Redundancy | 27.4 | |
CC(1/2) | 0.978 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 1.8M Ammonium sulfate, 0.1M BIS-TRIS pH6.5, 2% v/v polyethylene glycol monomethylether 550 |