7EWS

Crystal structure of arginine kinase (AK3) from the ciliate Paramecium tetraurelia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	70.109, 77.727, 162.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.420 - 2.000
R-factor	0.19874
Rwork	0.197
R-free	0.23685
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7EGZ
RMSD bond length	0.009
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.420	2.170
High resolution limit [Å]	2.000	2.030
Rmeas	0.330
Number of reflections	58371	20108
<I/σ(I)>	9
Completeness [%]	99.7
Redundancy	11.8
CC(1/2)		0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Hepes-NaOH, pH 7.0, 20% PEG4000