7EPR
Partial Consensus L-threonine 3-dehydrogenase (C-Change)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.019, 58.474, 138.130 |
Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
Resolution | 46.000 - 2.200 |
Rwork | 0.208 |
R-free | 0.25820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wmx |
RMSD bond length | 0.009 |
RMSD bond angle | 1.476 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.074 | 0.559 |
Number of reflections | 412432 | 8720 |
<I/σ(I)> | 16.3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 275 | 20% [w/v] PEG 3350, 0.2M sodium phosphate monobasic monohydrate |