7EN6
The crystal structure of Escherichia coli MurR in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-24 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.558, 77.583, 144.333 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.792 - 2.276 |
R-factor | 0.1929 |
Rwork | 0.189 |
R-free | 0.26330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ivn |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.310 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.079 | 0.568 |
Rmeas | 0.082 | 0.594 |
Rpim | 0.023 | 0.169 |
Number of reflections | 32333 | 1549 |
<I/σ(I)> | 27.7 | 2.6 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 12.5 | 11.8 |
CC(1/2) | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M L-proline, 0.1 M HEPES pH 7.5, 10% w/v polyethylene glycol 3,350 |