7EKX
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A E564Q) in complex with maltononaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 160.654, 206.280, 258.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.150 - 3.400 |
R-factor | 0.2739 |
Rwork | 0.272 |
R-free | 0.30340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d0f |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.160 | 3.490 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 58913 | 4334 |
<I/σ(I)> | 8.31 | |
Completeness [%] | 99.6 | |
Redundancy | 7.3 | |
CC(1/2) | 0.998 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride |