7EJZ

Complex Structure of antibody BD-503 and RBD-S477N of COVID-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	80.711, 145.915, 146.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.660 - 3.630
R-factor	0.2455
Rwork	0.241
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bwj
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.660	3.763
High resolution limit [Å]	3.630	3.633
Rmeas	0.469
Rpim	0.130	0.292
Number of reflections	10003	930
<I/σ(I)>	7.96
Completeness [%]	96.0	81.45
Redundancy	12.8	12.9
CC(1/2)	0.958	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Magnesium chloride hexahydrate, 0.1M Sodium citrate, pH 5.0, 15% (w/v) PEG 4000