7EJT
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A) in complex with maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97908 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.387, 199.268, 133.743 |
Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
Resolution | 40.480 - 3.200 |
R-factor | 0.2556 |
Rwork | 0.255 |
R-free | 0.26750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d0f |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.480 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.068 | |
Number of reflections | 66972 | 3348 |
<I/σ(I)> | 13.43 | |
Completeness [%] | 99.9 | |
Redundancy | 3.8 | |
CC(1/2) | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride |