7EHH

Crystal structure of alpha-glucosidase from Weissella cibaria BKK1 in complex with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2019-03-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 41 2 2
Unit cell lengths	116.031, 116.031, 106.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.180 - 2.000
R-factor	0.1609
Rwork	0.159
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d9b
RMSD bond length	0.009
RMSD bond angle	1.210
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmeas	0.229
Rpim	0.089
Number of reflections	67707	6686
<I/σ(I)>	19.51
Completeness [%]	99.6
Redundancy	6.2
CC(1/2)		0.249

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Ammonium sulfate, dioxane, MES