7EDR

The crystal structure of the FERM and C-terminal domain complex of Drosophila Merlin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 2 21 21
Unit cell lengths	86.464, 100.366, 163.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.976 - 2.527
R-factor	0.1866
Rwork	0.184
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrj
RMSD bond length	0.009
RMSD bond angle	0.933
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.527	2.530
Rpim	0.047
Number of reflections	48350	2354
<I/σ(I)>	14.2
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)		0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium cacodylate/Hydrochloric acid, pH 6.5, 1.0 M sodium citrate tribasic