7EAN

immune complex of SARS-CoV-2 RBD and cross-neutralizing antibody 6D6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	58.280, 85.340, 160.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.251 - 1.910
R-factor	0.1921
Rwork	0.191
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 1wej
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.080	1.960
High resolution limit [Å]	1.910	1.910
Rpim	2.700	0.913
Number of reflections	119757	6201
<I/σ(I)>	13.7
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)	0.999	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	0.2M sodium sulfocyanate, 20%PEG3350