7E6V
The crystal structure of foot-and-mouth disease virus(FMDV) 2C protein 97-318aa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979081 |
Spacegroup name | P 1 |
Unit cell lengths | 36.720, 40.737, 72.937 |
Unit cell angles | 77.22, 84.38, 89.96 |
Refinement procedure
Resolution | 38.480 - 1.832 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.24900 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.719 | 1.940 |
High resolution limit [Å] | 1.832 | 1.840 |
Rmerge | 0.069 | 0.618 |
Number of reflections | 67455 | 10786 |
<I/σ(I)> | 7.75 | 1.23 |
Completeness [%] | 93.5 | 93 |
Redundancy | 1.69 | 1.65 |
CC(1/2) | 0.996 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 296 | 0.09M Ammonium acetate, 0.045M Sodium citrate tribasic dihydrate pH 5.6, 13.5%w/v PEG 4000, 4% PEG P400, 5mM TECP |