7DWM
Crystal structure of the phage VqmA-DPO complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-11-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979106 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 133.664, 133.664, 89.514 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.430 - 2.650 |
R-factor | 0.2598 |
Rwork | 0.257 |
R-free | 0.29070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ide |
RMSD bond length | 0.006 |
RMSD bond angle | 1.113 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 115.760 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.068 | 0.922 |
Rmeas | 0.979 | |
Rpim | 0.323 | |
Number of reflections | 25381 | 2882 |
<I/σ(I)> | 18.4 | 2.3 |
Completeness [%] | 93.4 | |
Redundancy | 8.9 | |
CC(1/2) | 0.999 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M TRIS hydrochloride pH 7.2, 0.005 M Magnesium sulfate hydrate, 2.4 M 1,6-Hexanediol |