7DWH
Complex structure of SAM-dependent methyltransferase ribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-12-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.923, 77.305, 101.469 |
| Unit cell angles | 90.00, 93.24, 90.00 |
Refinement procedure
| Resolution | 29.534 - 3.100 |
| R-factor | 0.2114 |
| Rwork | 0.209 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dlz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.667 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.660 | 3.100 |
| Rmerge | 0.097 | 0.075 | 0.236 |
| Rmeas | 0.108 | 0.081 | 0.278 |
| Rpim | 0.045 | 0.032 | 0.143 |
| Total number of observations | 81837 | ||
| Number of reflections | 17380 | 1833 | 1595 |
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 97.7 | 99.7 | 90.9 |
| Redundancy | 4.7 | 6.3 | 2.9 |
| CC(1/2) | 0.996 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 291 | sodium phosphate monobasic monohydrate, polyethylene glycol 3350 |
