7DV7
Structure of a novel beta-mannanase BaMan113A from Bacillus sp. N16-5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.820, 109.145, 150.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.860 - 1.440 |
R-factor | 0.1506 |
Rwork | 0.150 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3civ |
RMSD bond length | 0.013 |
RMSD bond angle | 1.777 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.490 |
High resolution limit [Å] | 1.440 | 1.440 |
Number of reflections | 161843 | 13237 |
<I/σ(I)> | 57.1 | |
Completeness [%] | 97.3 | |
Redundancy | 8.2 | |
CC(1/2) | 0.973 | 0.986 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | Tacsimate |