7DTR

Structure of an AcrIF protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	77.689, 77.689, 145.121
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.840 - 2.100
R-factor	0.2158
Rwork	0.215
R-free	0.23330
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	15917	1545
<I/σ(I)>	16.8
Completeness [%]	99.3
Redundancy	29.2
CC(1/2)	0.998	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	2-propanol, imidazole pH 7.6