7DGK
The Co-bound dimeric structure of K78H/G80A/H82A myoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.202, 62.409, 82.553 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.080 - 1.750 |
R-factor | 0.20791 |
Rwork | 0.206 |
R-free | 0.25118 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vm9 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.752 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.081 | 1.378 |
Number of reflections | 29542 | 4248 |
<I/σ(I)> | 10.6 | 1.3 |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.679 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M Sodium acetate, 0.1 M Tris-HCl, 25% (w/v) PEG 6,000 |