7D2C
The Crystal Structure of human PARP14 from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97936 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.761, 41.281, 109.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 1.560 |
Rwork | 0.175 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5qi5 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.235 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.766 | 1.590 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.053 | 0.137 |
Number of reflections | 22373 | 1006 |
<I/σ(I)> | 23.1 | |
Completeness [%] | 99.0 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NaCl, 0.1M Tris pH8.5, 25% PEG3350 |