7CRG

Beta-strand-mediated dimeric formation of the extended Ig-like domains of human lamin A/C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-10-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 64 2 2
Unit cell lengths	92.648, 92.648, 205.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.380 - 1.800
R-factor	0.1942
Rwork	0.193
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ifr
RMSD bond length	0.007
RMSD bond angle	0.823
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rpim		0.073
Number of reflections	47954	47954
<I/σ(I)>	34.2
Completeness [%]	98.1
Redundancy	24.5
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.2 M Potassium Chloride, 0.05 M HEPES pH 7.5, 35%(v/v) Pentaerythritol Propoxylate (5/4PO/OH)