7CQD
The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA14 tag inserted between the residues 235 and 236
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.360, 80.175, 168.540 |
Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.990 | 3.360 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.115 | 0.965 |
Number of reflections | 70558 | 4745 |
<I/σ(I)> | 8 | 1.2 |
Completeness [%] | 99.5 | |
Redundancy | 3.4 | |
CC(1/2) | 0.994 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10%(wt/vol) PEG 3350, 0.2 M L-proline, 0.1 M HEPES-Na (pH 7.5) |