7CQC
The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA14 tag inserted between the residues 181 and 184
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.358, 75.203, 172.533 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.680 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.093 | 1.469 |
Number of reflections | 47746 | 2685 |
<I/σ(I)> | 13.1 | 1.5 |
Completeness [%] | 99.9 | |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20%(wt./vol.) PEG 3350, 0.2 M potassium sodium tartrate |