7C3A
Ferredoxin reductase in carbazole 1,9a-dioxygenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 158.692, 158.692, 81.446 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.670 - 2.600 |
R-factor | 0.2509 |
Rwork | 0.249 |
R-free | 0.29150 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.284 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | SOLVE |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.100 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.088 | 0.408 |
Number of reflections | 32288 | 3208 |
<I/σ(I)> | 8.7 | 3.8 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 5.9 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES | |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES | |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 mM calcium acetate or magnesium acetate, 8-12%(w/v) PEG 8000, 0.2 M sodium iodide, 0.08 M MES |