7BWQ

Structure of nonstructural protein Nsp9 from SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	C 2 2 21
Unit cell lengths	88.713, 134.445, 166.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.487 - 2.954
R-factor	0.2168
Rwork	0.213
R-free	0.29890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qz8
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.040
High resolution limit [Å]	2.950	6.750	2.950
Rmerge	0.221	0.197	0.593
Rmeas	0.231	0.206	0.618
Rpim	0.066	0.060	0.170
Total number of observations	275658
Number of reflections	21270	1880	1762
<I/σ(I)>	6.4
Completeness [%]	100.0	99.8	99.9
Redundancy	13	12.3	13.3
CC(1/2)		0.915	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3	293	100mM Citric Acid, pH 3.0, 200mM Potassium sodium tartrate tetrahydrate , 2.0M (NH4)2SO4