7BRL
Atrial Natriuretic Peptide Receptor complexed with Deletion mutant of rat Atrial Natriuretic Peptide[4-17,23]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-28 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 |
Unit cell lengths | 100.273, 100.273, 270.415 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.517 - 3.200 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dp4 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.822 |
Data reduction software | HKL-2000 (716.1) |
Data scaling software | HKL-2000 (716.1) |
Phasing software | MOLREP (11.2.08) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.290 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.048 | 0.373 |
Number of reflections | 24713 | 2096 |
<I/σ(I)> | 27.6 | 3.5 |
Completeness [%] | 97.4 | |
Redundancy | 23.1 | |
CC(1/2) | 0.990 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | Sodium malonate, MES |