7BHF
DARPin_D5/Her3 domain 4 complex, orthorhombic crystals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.258, 64.951, 144.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.190 - 1.997 |
R-factor | 0.2003 |
Rwork | 0.198 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bhe |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3 (18-SEP-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.192 | 2.031 |
High resolution limit [Å] | 1.997 | 1.997 |
Rmerge | 0.216 | 1.657 |
Rmeas | 0.230 | 1.776 |
Rpim | 0.075 | 0.610 |
Number of reflections | 38572 | 1904 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 94.7 | 96.5 |
Redundancy | 8.87 | 8.2 |
CC(1/2) | 0.991 | 0.387 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.74 | 293 | 0.1 M Na citrate, 10% PEG4000, 0.2 M Li sulfate |