7BBN

Crystal structure of an ancient sequence-reconstructed Elongation Factor Tu (node 317)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.918401
Spacegroup name	C 2 2 21
Unit cell lengths	97.342, 100.289, 160.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.440 - 1.680
R-factor	0.1927
Rwork	0.191
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
RMSD bond length	0.013
RMSD bond angle	1.851
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.440	29.420	1.710
High resolution limit [Å]	1.680	9.050	1.680
Rmerge	0.093	0.062	0.834
Rmeas	0.098	0.066	0.878
Rpim	0.029	0.021	0.268
Total number of observations		5821	37728
Number of reflections	88432	632	3787
<I/σ(I)>	14.1	33.9	2.5
Completeness [%]	98.9	97.2	83.2
Redundancy	11	9.2	10
CC(1/2)	0.999	0.997	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.2 M Lithium citrate tribasic tetrathydrate, 20% w/v PEG 3350, pH 8.4