7B55
Crystal structure of CaMKII-actinin complex bound to MES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9119 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.574, 70.023, 91.319 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.570 - 1.600 |
R-factor | 0.1979 |
Rwork | 0.197 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vz6 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.807 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.320 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rpim | 0.018 | 0.783 |
Number of reflections | 57062 | 2778 |
<I/σ(I)> | 14.6 | 0.8 |
Completeness [%] | 100.0 | |
Redundancy | 12.6 | |
CC(1/2) | 1.000 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M MES pH 6.0, 20% w/v PEG4000, 0.2 M Lithium Sulfate |