7ABA
The structure of the Bottromycin biosynthetic protein SalCYP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.738 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.448, 97.403, 93.285 |
Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
Resolution | 48.825 - 1.850 |
R-factor | 0.143185800858 |
Rwork | 0.141 |
R-free | 0.17574 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.779 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.825 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.158 | 0.699 |
Number of reflections | 74132 | 7163 |
<I/σ(I)> | 18.9 | |
Completeness [%] | 99.6 | |
Redundancy | 28.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG3350, Calcium acetate |