6ZYV

Structure of a Plasmodium PIR protein ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97166
Spacegroup name	P 21 21 21
Unit cell lengths	60.170, 63.608, 199.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.840 - 2.150
R-factor	0.2129
Rwork	0.212
R-free	0.23660
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.840	2.220
High resolution limit [Å]	2.150	2.150
Rpim	0.040	0.632
Number of reflections	42523	3621
<I/σ(I)>	4.8	1.3
Completeness [%]	99.8
Redundancy	1.9
CC(1/2)	1.000	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M HEPES pH 6.5 45% w/v Poly(acrylic acid sodium salt) 2100