6ZTR

Crystal Structure of the anti-human P-Cadherin Fab CQY684 in complex with human P-Cadherin(108-324)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.999990
Spacegroup name	P 21 21 2
Unit cell lengths	172.691, 77.795, 133.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.490 - 2.100
R-factor	0.1936
Rwork	0.192
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ztf 6ztb
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.490	72.490	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.199	0.039	2.392
Rmeas	0.216	0.043	2.585
Total number of observations	707980
Number of reflections	105445	1337	7725
<I/σ(I)>	7.97	33.36	0.88
Completeness [%]	100.0	99.3	100
Redundancy	6.714	6.073	6.967
CC(1/2)	0.995	0.995	0.396

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M calcium acetate, 10% (w/v) PEG 8,000, 0.1M MES pH 6.5