6ZQH

Yeast Uba1 in complex with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 2 21 21
Unit cell lengths	76.868, 173.172, 214.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.158 - 2.032
R-factor	0.1959
Rwork	0.195
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmm
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.160	2.105
High resolution limit [Å]	2.026	2.026
Number of reflections	131321	6564
<I/σ(I)>	6.5
Completeness [%]	70.4
Redundancy	5.6
CC(1/2)	0.990	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium formate, PEG 3350