6ZFF
Pyrococcus furiosus Rad50 coiled coils in rod configuration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-04 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.280980 |
Spacegroup name | I 41 |
Unit cell lengths | 128.300, 128.300, 74.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.370 - 3.000 |
R-factor | 0.254 |
Rwork | 0.250 |
R-free | 0.29470 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.319 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.370 | 3.108 |
High resolution limit [Å] | 3.000 | 3.001 |
Number of reflections | 12199 | 1216 |
<I/σ(I)> | 18.71 | |
Completeness [%] | 98.4 | |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 2.25 M ammonium sulfate and 50 mM Tris/HCl pH 8 |