6ZD6
Structure of apo telomerase from Candida Tropicalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.980109 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.695, 65.695, 387.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.770 - 2.640 |
R-factor | 0.2222 |
Rwork | 0.220 |
R-free | 0.27250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zd1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.624 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.770 | 2.736 |
High resolution limit [Å] | 2.640 | 2.642 |
Rmerge | 0.177 | 2.347 |
Rmeas | 0.183 | 2.432 |
Rpim | 0.048 | 0.635 |
Number of reflections | 47515 | 225 |
<I/σ(I)> | 10.99 | 1.3 |
Completeness [%] | 83.2 | 48.8 |
Redundancy | 14.5 | 14.8 |
CC(1/2) | 0.998 | 0.575 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.1 | 298 | 0.2 M sodium citrate tribasic dihydrate 0.1 M Bis-Tris propane (pH 6.1) 15% PEG3350 |