6YI5
In-situ structure of the trimeric HEF from influenza C by flexible fitting into a cryo-ET map.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 9.1 |
| Software | Dynamo,Coot,RELION,CTFFIND,NOVACTF,MDFF |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING ONLY |
