6YA9
Crystal structure of rsGCaMP in the ON state (non-illuminated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000029 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.430, 119.430, 96.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.820 - 2.150 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.820 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 38514 | 2816 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.2 | 15 |
| CC(1/2) | 0.999 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.21 M sodium formate, 0.1 M Bis-Tris-Propane buffer pH 8.5, 18% (w/v) PEG 3350 |
