6XZM
Arabidopsis UV-B photoreceptor UVR8 mutant D96N D107N W285A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-31 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 98.266, 98.266, 138.836 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.130 - 2.100 |
R-factor | 0.199549219029 |
Rwork | 0.197 |
R-free | 0.24813 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d9s |
RMSD bond length | 0.011 |
RMSD bond angle | 1.141 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.130 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 45831 | 4521 |
<I/σ(I)> | 13.84 | |
Completeness [%] | 100.0 | |
Redundancy | 20.1 | |
CC(1/2) | 0.999 | 0.483 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 22% PEG3350, 0.2M NaNO3, 5mg/mL. 20% PEG400 cryoprotectant |